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Image Search Results
Journal: PLoS ONE
Article Title: BAR Proteins PSTPIP1/2 Regulate Podosome Dynamics and the Resorption Activity of Osteoclasts
doi: 10.1371/journal.pone.0164829
Figure Lengend Snippet: Osteoclasts were grown on glass coverslips and transfected with constructs to express mRFP-tagged actin-binding domain of Ezrin (mRFP-ABDE) together with the GFP-PI3,4,5 P3 binding domain of Akt kinase or mRFP-Ezrin actin-binding domain and GFP-PSTPIP1 or GFP-PSTPIP1 and mRFP-PSTPIP2. A Individual podosomes were then observed by time-lapse videomicroscopy 48 hours after transfection (100 msec. per frame, 1 frame per 2 sec., , and Movies). B The fluorescence intensities represent the variations of mean fluorescence intensity associated to 35 podosomes in three independent experiments.
Article Snippet: HeLa cells seeded at a density 3x10 4 cells/mL were transfected with a mixture of 1μg plasmid DNA and 3μL
Techniques: Transfection, Construct, Binding Assay, Fluorescence