polyethylenimine reagent Search Results


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PEQLAB cationic polyethylenimine transfection reagent jetpei tm
Osteoclasts were grown on glass coverslips and transfected with constructs to express mRFP-tagged actin-binding domain of Ezrin (mRFP-ABDE) together with the GFP-PI3,4,5 P3 binding domain of Akt kinase or mRFP-Ezrin actin-binding domain and GFP-PSTPIP1 or GFP-PSTPIP1 and mRFP-PSTPIP2. A Individual podosomes were then observed by time-lapse videomicroscopy 48 hours after <t>transfection</t> (100 msec. per frame, 1 frame per 2 sec., , and Movies). B The fluorescence intensities represent the variations of mean fluorescence intensity associated to 35 podosomes in three independent experiments.
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Osteoclasts were grown on glass coverslips and transfected with constructs to express mRFP-tagged actin-binding domain of Ezrin (mRFP-ABDE) together with the GFP-PI3,4,5 P3 binding domain of Akt kinase or mRFP-Ezrin actin-binding domain and GFP-PSTPIP1 or GFP-PSTPIP1 and mRFP-PSTPIP2. A Individual podosomes were then observed by time-lapse videomicroscopy 48 hours after <t>transfection</t> (100 msec. per frame, 1 frame per 2 sec., , and Movies). B The fluorescence intensities represent the variations of mean fluorescence intensity associated to 35 podosomes in three independent experiments.
Polyethylenimine Transfection Reagent, supplied by Merck KGaA, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Osteoclasts were grown on glass coverslips and transfected with constructs to express mRFP-tagged actin-binding domain of Ezrin (mRFP-ABDE) together with the GFP-PI3,4,5 P3 binding domain of Akt kinase or mRFP-Ezrin actin-binding domain and GFP-PSTPIP1 or GFP-PSTPIP1 and mRFP-PSTPIP2. A Individual podosomes were then observed by time-lapse videomicroscopy 48 hours after <t>transfection</t> (100 msec. per frame, 1 frame per 2 sec., , and Movies). B The fluorescence intensities represent the variations of mean fluorescence intensity associated to 35 podosomes in three independent experiments.
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Osteoclasts were grown on glass coverslips and transfected with constructs to express mRFP-tagged actin-binding domain of Ezrin (mRFP-ABDE) together with the GFP-PI3,4,5 P3 binding domain of Akt kinase or mRFP-Ezrin actin-binding domain and GFP-PSTPIP1 or GFP-PSTPIP1 and mRFP-PSTPIP2. A Individual podosomes were then observed by time-lapse videomicroscopy 48 hours after <t>transfection</t> (100 msec. per frame, 1 frame per 2 sec., , and Movies). B The fluorescence intensities represent the variations of mean fluorescence intensity associated to 35 podosomes in three independent experiments.
11 Polyethylenimine Reagent, supplied by Polysciences inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Osteoclasts were grown on glass coverslips and transfected with constructs to express mRFP-tagged actin-binding domain of Ezrin (mRFP-ABDE) together with the GFP-PI3,4,5 P3 binding domain of Akt kinase or mRFP-Ezrin actin-binding domain and GFP-PSTPIP1 or GFP-PSTPIP1 and mRFP-PSTPIP2. A Individual podosomes were then observed by time-lapse videomicroscopy 48 hours after <t>transfection</t> (100 msec. per frame, 1 frame per 2 sec., , and Movies). B The fluorescence intensities represent the variations of mean fluorescence intensity associated to 35 podosomes in three independent experiments.
Polyethylenimines Transfection Reagent Fugene® Hd, supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Osteoclasts were grown on glass coverslips and transfected with constructs to express mRFP-tagged actin-binding domain of Ezrin (mRFP-ABDE) together with the GFP-PI3,4,5 P3 binding domain of Akt kinase or mRFP-Ezrin actin-binding domain and GFP-PSTPIP1 or GFP-PSTPIP1 and mRFP-PSTPIP2. A Individual podosomes were then observed by time-lapse videomicroscopy 48 hours after <t>transfection</t> (100 msec. per frame, 1 frame per 2 sec., , and Movies). B The fluorescence intensities represent the variations of mean fluorescence intensity associated to 35 podosomes in three independent experiments.
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PolyScience polyethylenimine reagent (pei
Osteoclasts were grown on glass coverslips and transfected with constructs to express mRFP-tagged actin-binding domain of Ezrin (mRFP-ABDE) together with the GFP-PI3,4,5 P3 binding domain of Akt kinase or mRFP-Ezrin actin-binding domain and GFP-PSTPIP1 or GFP-PSTPIP1 and mRFP-PSTPIP2. A Individual podosomes were then observed by time-lapse videomicroscopy 48 hours after <t>transfection</t> (100 msec. per frame, 1 frame per 2 sec., , and Movies). B The fluorescence intensities represent the variations of mean fluorescence intensity associated to 35 podosomes in three independent experiments.
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Image Search Results


Osteoclasts were grown on glass coverslips and transfected with constructs to express mRFP-tagged actin-binding domain of Ezrin (mRFP-ABDE) together with the GFP-PI3,4,5 P3 binding domain of Akt kinase or mRFP-Ezrin actin-binding domain and GFP-PSTPIP1 or GFP-PSTPIP1 and mRFP-PSTPIP2. A Individual podosomes were then observed by time-lapse videomicroscopy 48 hours after transfection (100 msec. per frame, 1 frame per 2 sec., , and Movies). B The fluorescence intensities represent the variations of mean fluorescence intensity associated to 35 podosomes in three independent experiments.

Journal: PLoS ONE

Article Title: BAR Proteins PSTPIP1/2 Regulate Podosome Dynamics and the Resorption Activity of Osteoclasts

doi: 10.1371/journal.pone.0164829

Figure Lengend Snippet: Osteoclasts were grown on glass coverslips and transfected with constructs to express mRFP-tagged actin-binding domain of Ezrin (mRFP-ABDE) together with the GFP-PI3,4,5 P3 binding domain of Akt kinase or mRFP-Ezrin actin-binding domain and GFP-PSTPIP1 or GFP-PSTPIP1 and mRFP-PSTPIP2. A Individual podosomes were then observed by time-lapse videomicroscopy 48 hours after transfection (100 msec. per frame, 1 frame per 2 sec., , and Movies). B The fluorescence intensities represent the variations of mean fluorescence intensity associated to 35 podosomes in three independent experiments.

Article Snippet: HeLa cells seeded at a density 3x10 4 cells/mL were transfected with a mixture of 1μg plasmid DNA and 3μL cationic polyethylenimine transfection reagent JetPEI TM (Peqlab Biotechnologie GmbH).

Techniques: Transfection, Construct, Binding Assay, Fluorescence